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KMID : 1123920030170051305
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 5 p.1305 ~ p.1308
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Effect of Salviae Miltiorrhzae Radix on Cultured Spinal Dorsal Root Ganglion Neurons Damaged by Reactive Oxygen Species
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Seo Eun-A
Choi Yu-Sun
Yang Hyun-Woong
Lee Kang-Chang
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Abstract
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To evaluate the neurotoxicity of reactive oxygen species (ROS) in cultured cultured spinal dorsal root(DRG) neurons derived from neonatal mouse, Cytotoxicity was measured by MTS assay after cultured cells were grown for 3 hours in the media containing 1～60 μM hydrogen peroxide (H₂O₂). In addition the neuroprotective effect of Salviae Miltiorrhzae Radix (SMR) was measured in these cultrures. Cell viability was positively decreased in a dose- and time-dependent manner after exposure of cultured mouse DRG neurons to 30 tt M H202 for 3 hours. In the neuroprotective effect of SMR on H₂O₂-mediated toxicity, SMR prevented the H₂O₂-induced neurotoxicity in these cultures. From these results. it suggests that H₂0₂ is toxic in cultured mouse spinal motor neurons and selective herb extract such as Uncariae Ramulus Cum Uncis is effective in prevetion of the neurotoxicity induced by H₂O₂.
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KEYWORD
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ROS, Salviae Miltiorrhzae Radix, Spinal dorsal root ganglion neuron
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